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Tunable translational control using site-specific unnatural amino acid incorporation in Escherichia coli
Translation of target gene transcripts in Escherichia coli harboring UAG amber stop codons can be switched on by the amber-codon-specific incorporation of an exogenously supplied unnatural amino acid, 3-iodo-(L)-tyrosine. Here, we report that this translational switch can control the translational e...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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PeerJ Inc.
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4419535/ https://www.ncbi.nlm.nih.gov/pubmed/25945307 http://dx.doi.org/10.7717/peerj.904 |