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Differential Requirements of Singleplex and Multiplex Recombineering of Large DNA Constructs
Recombineering is an in vivo genetic engineering technique involving homologous recombination mediated by phage recombination proteins. The use of recombineering methodology is not limited by size and sequence constraints and therefore has enabled the streamlined construction of bacterial strains an...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425527/ https://www.ncbi.nlm.nih.gov/pubmed/25954970 http://dx.doi.org/10.1371/journal.pone.0125533 |