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Differential Requirements of Singleplex and Multiplex Recombineering of Large DNA Constructs

Recombineering is an in vivo genetic engineering technique involving homologous recombination mediated by phage recombination proteins. The use of recombineering methodology is not limited by size and sequence constraints and therefore has enabled the streamlined construction of bacterial strains an...

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Detalles Bibliográficos
Autores principales:	Reddy, Thimma R., Kelsall, Emma J., Fevat, Léna M. S., Munson, Sarah E., Cowley, Shaun M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4425527/ https://www.ncbi.nlm.nih.gov/pubmed/25954970 http://dx.doi.org/10.1371/journal.pone.0125533

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