Redesigning Protein Cavities as a Strategy for Increasing Affinity in Protein-Protein Interaction: Interferon-γ Receptor 1 as a Model

Combining computational and experimental tools, we present a new strategy for designing high affinity variants of a binding protein. The affinity is increased by mutating residues not at the interface, but at positions lining internal cavities of one of the interacting molecules. Filling the cavitie...

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Detalles Bibliográficos
Autores principales: Černý, Jiří, Biedermannová, Lada, Mikulecký, Pavel, Zahradník, Jiří, Charnavets, Tatsiana, Šebo, Peter, Schneider, Bohdan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427845/
https://www.ncbi.nlm.nih.gov/pubmed/26060819
http://dx.doi.org/10.1155/2015/716945

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427845/
https://www.ncbi.nlm.nih.gov/pubmed/26060819
http://dx.doi.org/10.1155/2015/716945

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