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Deriving accurate microbiota profiles from human samples with low bacterial content through post-sequencing processing of Illumina MiSeq data

BACKGROUND: The rapid expansion of 16S rRNA gene sequencing in challenging clinical contexts has resulted in a growing body of literature of variable quality. To a large extent, this is due to a failure to address spurious signal that is characteristic of samples with low levels of bacteria and high...

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Detalles Bibliográficos
Autores principales: Jervis-Bardy, Jake, Leong, Lex E X, Marri, Shashikanth, Smith, Renee J, Choo, Jocelyn M, Smith-Vaughan, Heidi C, Nosworthy, Elizabeth, Morris, Peter S, O’Leary, Stephen, Rogers, Geraint B, Marsh, Robyn L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4428251/
https://www.ncbi.nlm.nih.gov/pubmed/25969736
http://dx.doi.org/10.1186/s40168-015-0083-8