In-Cell RNA Hydrolysis Assay: A Method for the Determination of the RNase Activity of Potential RNases

Conventional procedures to assay RNA degradation by a protein with ribonuclease (RNase) activity require a step to isolate intact RNA molecules, which are used as a substrate. Here, we established a novel “In-cell RNA hydrolysis assay” in which RNAs within cells are used as a substrate for the RNA-h...

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Detalles Bibliográficos
Autores principales: Seo, Youngsil, Jun, Hye-Ryeong, Lee, Joungmin, Park, Hyunjoon, Kim, Minjae, Lee, Youngsoo, Kwon, Myung-Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432088/
https://www.ncbi.nlm.nih.gov/pubmed/25632893
http://dx.doi.org/10.1007/s12033-015-9844-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432088/
https://www.ncbi.nlm.nih.gov/pubmed/25632893
http://dx.doi.org/10.1007/s12033-015-9844-7

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