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In-Cell RNA Hydrolysis Assay: A Method for the Determination of the RNase Activity of Potential RNases
Conventional procedures to assay RNA degradation by a protein with ribonuclease (RNase) activity require a step to isolate intact RNA molecules, which are used as a substrate. Here, we established a novel “In-cell RNA hydrolysis assay” in which RNAs within cells are used as a substrate for the RNA-h...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer US
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432088/ https://www.ncbi.nlm.nih.gov/pubmed/25632893 http://dx.doi.org/10.1007/s12033-015-9844-7 |