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Characterization of genome-wide ordered sequence-tagged Mycobacterium mutant libraries by Cartesian Pooling-Coordinate Sequencing

Reverse genetics research approaches require the availability of methods to rapidly generate specific mutants. Alternatively, where these methods are lacking, the construction of pre-characterized libraries of mutants can be extremely valuable. However, this can be complex, expensive and time consum...

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Detalles Bibliográficos
Autores principales: Vandewalle, Kristof, Festjens, Nele, Plets, Evelyn, Vuylsteke, Marnik, Saeys, Yvan, Callewaert, Nico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432585/
https://www.ncbi.nlm.nih.gov/pubmed/25960123
http://dx.doi.org/10.1038/ncomms8106