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Characterization of genome-wide ordered sequence-tagged Mycobacterium mutant libraries by Cartesian Pooling-Coordinate Sequencing
Reverse genetics research approaches require the availability of methods to rapidly generate specific mutants. Alternatively, where these methods are lacking, the construction of pre-characterized libraries of mutants can be extremely valuable. However, this can be complex, expensive and time consum...
Autores principales: | Vandewalle, Kristof, Festjens, Nele, Plets, Evelyn, Vuylsteke, Marnik, Saeys, Yvan, Callewaert, Nico |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432585/ https://www.ncbi.nlm.nih.gov/pubmed/25960123 http://dx.doi.org/10.1038/ncomms8106 |
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