Expression of heterologous sigma factors enables functional screening of metagenomic and heterologous genomic libraries

A key limitation in using heterologous genomic or metagenomic libraries in functional genomics and genome engineering is the low expression of heterologous genes in screening hosts, such as Escherichia coli. To overcome this limitation, here we generate E. coli strains capable of recognizing heterol...

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Detalles Bibliográficos
Autores principales: Gaida, Stefan M., Sandoval, Nicholas R., Nicolaou, Sergios A., Chen, Yili, Venkataramanan, Keerthi P., Papoutsakis, Eleftherios T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432631/
https://www.ncbi.nlm.nih.gov/pubmed/25944046
http://dx.doi.org/10.1038/ncomms8045

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432631/
https://www.ncbi.nlm.nih.gov/pubmed/25944046
http://dx.doi.org/10.1038/ncomms8045

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