Efficient expression of codon-adapted affinity tagged super folder green fluorescent protein for synchronous protein localization and affinity purification studies in Tetrahymena thermophila

BACKGROUND: A superior Green Fluorescent Protein (GFP) mutant, known as superfolder GFP (sfGFP), is more soluble, faster folding, and is the brightest of the known GFP mutants. This study aimed to create a codon-adapted sfGFP tag (TtsfGFP) for simultaneous protein localization and affinity purificat...

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Detalles Bibliográficos
Autores principales: Yilmaz, Gürkan, Arslanyolu, Muhittin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432788/
https://www.ncbi.nlm.nih.gov/pubmed/25887423
http://dx.doi.org/10.1186/s12896-015-0137-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432788/
https://www.ncbi.nlm.nih.gov/pubmed/25887423
http://dx.doi.org/10.1186/s12896-015-0137-9

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