Frequent problems and their resolutions by using thermal asymmetric interlaced PCR (TAIL-PCR) to clone genes in Arabidopsis T-DNA tagged mutants

T-DNA insertional mutagenesis is a powerful tool in Arabidopsis functional genomics research. Previous studies have developed thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) as an efficient strategy in isolation of DNA sequences adjacent to known sequences in T-DNA tagged mutants....

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Detalles Bibliográficos
Autores principales: Wu, Lei, Di, Dong-Wei, Zhang, Dan, Song, Bin, Luo, Pan, Guo, Guang-Qin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Articles; Agriculture and Environmental Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433792/
https://www.ncbi.nlm.nih.gov/pubmed/26019639
http://dx.doi.org/10.1080/13102818.2014.998161

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433792/
https://www.ncbi.nlm.nih.gov/pubmed/26019639
http://dx.doi.org/10.1080/13102818.2014.998161

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