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Lactic acid microflora of the gut of snail Cornu aspersum

The intestinal lactic acid microflora of the edible snail Cornu aspersum was studied by culture-based methods and was phenotypically and molecularly characterized. The antibacterial activity of lactic acid bacteria (LAB) isolates was investigated. Snails in different stages of development were colle...

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Autores principales: Koleva, Zdravka, Dedov, Ivaylo, Kizheva, Joana, Lipovanska, Roxana, Moncheva, Penka, Hristova, Petya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433887/
https://www.ncbi.nlm.nih.gov/pubmed/26019550
http://dx.doi.org/10.1080/13102818.2014.947071
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author Koleva, Zdravka
Dedov, Ivaylo
Kizheva, Joana
Lipovanska, Roxana
Moncheva, Penka
Hristova, Petya
author_facet Koleva, Zdravka
Dedov, Ivaylo
Kizheva, Joana
Lipovanska, Roxana
Moncheva, Penka
Hristova, Petya
author_sort Koleva, Zdravka
collection PubMed
description The intestinal lactic acid microflora of the edible snail Cornu aspersum was studied by culture-based methods and was phenotypically and molecularly characterized. The antibacterial activity of lactic acid bacteria (LAB) isolates was investigated. Snails in different stages of development were collected from farms located in several regions of Bulgaria. One hundred twenty-two isolates, belonging to the group of LAB, were characterized morphologically and were divided into four groups. Representative isolates from each morphological type were subjected to phenotypic characterization and molecular identification. The snail gut lactic acid microflora was composed by Enterococcus (17 isolates), Lactococcus (12 isolates), Leuconostoc (7 isolates), Lactobacillus (18 isolates) and Weissella (1 isolate). The species affiliation of Lactococcus lactis (12), Leuconostoc mesenteroides (4) and Lactobacillus plantarum (2) was confirmed by species-specific primers. The Lactobacillus isolates were identified by sequence analysis of 16S rDNA as Lactobacillus brevis (12), L. plantarum (2), Lactobacillus graminis (1) and Lactobacillus curvatus (3). The species L. brevis, L. graminis and L. curvatus were found in snails in a phase of hibernation, whereas L. plantarum was identified both in active and hibernation phases. Antibacterial activity (bacteriocine-like) was shown only by one strain of L. mesentereoides P4/8 against Propionibacterium acnes. The present study showed that the LAB are a component of the microbial communities in the snail digestive system. This is the first report on Lactobacillus strains detected in the gut of C. aspersum.
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spelling pubmed-44338872015-05-25 Lactic acid microflora of the gut of snail Cornu aspersum Koleva, Zdravka Dedov, Ivaylo Kizheva, Joana Lipovanska, Roxana Moncheva, Penka Hristova, Petya Biotechnol Biotechnol Equip Article; Agriculture and Environmental Biotechnology The intestinal lactic acid microflora of the edible snail Cornu aspersum was studied by culture-based methods and was phenotypically and molecularly characterized. The antibacterial activity of lactic acid bacteria (LAB) isolates was investigated. Snails in different stages of development were collected from farms located in several regions of Bulgaria. One hundred twenty-two isolates, belonging to the group of LAB, were characterized morphologically and were divided into four groups. Representative isolates from each morphological type were subjected to phenotypic characterization and molecular identification. The snail gut lactic acid microflora was composed by Enterococcus (17 isolates), Lactococcus (12 isolates), Leuconostoc (7 isolates), Lactobacillus (18 isolates) and Weissella (1 isolate). The species affiliation of Lactococcus lactis (12), Leuconostoc mesenteroides (4) and Lactobacillus plantarum (2) was confirmed by species-specific primers. The Lactobacillus isolates were identified by sequence analysis of 16S rDNA as Lactobacillus brevis (12), L. plantarum (2), Lactobacillus graminis (1) and Lactobacillus curvatus (3). The species L. brevis, L. graminis and L. curvatus were found in snails in a phase of hibernation, whereas L. plantarum was identified both in active and hibernation phases. Antibacterial activity (bacteriocine-like) was shown only by one strain of L. mesentereoides P4/8 against Propionibacterium acnes. The present study showed that the LAB are a component of the microbial communities in the snail digestive system. This is the first report on Lactobacillus strains detected in the gut of C. aspersum. Taylor & Francis 2014-07-04 2014-10-21 /pmc/articles/PMC4433887/ /pubmed/26019550 http://dx.doi.org/10.1080/13102818.2014.947071 Text en © 2014 The Author(s). Published by Taylor & Francis. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Article; Agriculture and Environmental Biotechnology
Koleva, Zdravka
Dedov, Ivaylo
Kizheva, Joana
Lipovanska, Roxana
Moncheva, Penka
Hristova, Petya
Lactic acid microflora of the gut of snail Cornu aspersum
title Lactic acid microflora of the gut of snail Cornu aspersum
title_full Lactic acid microflora of the gut of snail Cornu aspersum
title_fullStr Lactic acid microflora of the gut of snail Cornu aspersum
title_full_unstemmed Lactic acid microflora of the gut of snail Cornu aspersum
title_short Lactic acid microflora of the gut of snail Cornu aspersum
title_sort lactic acid microflora of the gut of snail cornu aspersum
topic Article; Agriculture and Environmental Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4433887/
https://www.ncbi.nlm.nih.gov/pubmed/26019550
http://dx.doi.org/10.1080/13102818.2014.947071
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