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Exploring specific primers targeted against different genes for a multiplex PCR for detection of Listeria monocytogenes
The efficacy of six different sets of primers targeted against 16S rRNA and virulence genes such as ‘iap’, ‘hly’ and ‘prf’ was evaluated in separate PCR assays. The primer pairs targeted against 16S rRNA resulted into amplification of 1.2 kb PCR product. However, sets of primers targeted against dif...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434410/ https://www.ncbi.nlm.nih.gov/pubmed/28324291 http://dx.doi.org/10.1007/s13205-014-0225-x |