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Exploring specific primers targeted against different genes for a multiplex PCR for detection of Listeria monocytogenes

The efficacy of six different sets of primers targeted against 16S rRNA and virulence genes such as ‘iap’, ‘hly’ and ‘prf’ was evaluated in separate PCR assays. The primer pairs targeted against 16S rRNA resulted into amplification of 1.2 kb PCR product. However, sets of primers targeted against dif...

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Detalles Bibliográficos
Autores principales: Kumar, Ashwani, Grover, Sunita, Batish, Virender Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4434410/
https://www.ncbi.nlm.nih.gov/pubmed/28324291
http://dx.doi.org/10.1007/s13205-014-0225-x