De novo protein structure determination from near-atomic resolution cryo-EM maps

We present a de novo model building approach that combines predicted backbone conformations with side-chain density-fit to accurately assign sequence into density maps. We show this method yields accurate models for six experimental maps at 3.3–4.8 Å resolution, and produces a nearly complete model...

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Detalles Bibliográficos
Autores principales: Wang, Ray Yu-Ruei, Kudryashev, Mikhail, Li, Xueming, Egelman, Edward H., Basler, Marek, Cheng, Yifan, Baker, David, DiMaio, Frank
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435692/
https://www.ncbi.nlm.nih.gov/pubmed/25707029
http://dx.doi.org/10.1038/nmeth.3287

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435692/
https://www.ncbi.nlm.nih.gov/pubmed/25707029
http://dx.doi.org/10.1038/nmeth.3287

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