Cargando…

The Role of SUMO-Conjugating Enzyme Ubc9 in the Neuroprotection of Isoflurane Preconditioning Against Ischemic Neuronal Injury

Preconditioning with volatile anesthetics can create an ischemia tolerance against cerebral ischemia-reperfusion injury. We investigated whether ubiquitin conjugase 9 (Ubc9), the E2 conjugase for SUMOylation, is associated with neuroprotection induced by isoflurane preconditioning (IsoPC). In vitro,...

Descripción completa

Detalles Bibliográficos
Autores principales: Tong, Li, Wu, Zhixin, Ran, Mingzi, Chen, Yu, Yang, Lujia, Zhang, Haopeng, Zhang, Lina, Dong, Hailong, Xiong, Lize
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435903/
https://www.ncbi.nlm.nih.gov/pubmed/24961570
http://dx.doi.org/10.1007/s12035-014-8797-3
_version_ 1782371974634274816
author Tong, Li
Wu, Zhixin
Ran, Mingzi
Chen, Yu
Yang, Lujia
Zhang, Haopeng
Zhang, Lina
Dong, Hailong
Xiong, Lize
author_facet Tong, Li
Wu, Zhixin
Ran, Mingzi
Chen, Yu
Yang, Lujia
Zhang, Haopeng
Zhang, Lina
Dong, Hailong
Xiong, Lize
author_sort Tong, Li
collection PubMed
description Preconditioning with volatile anesthetics can create an ischemia tolerance against cerebral ischemia-reperfusion injury. We investigated whether ubiquitin conjugase 9 (Ubc9), the E2 conjugase for SUMOylation, is associated with neuroprotection induced by isoflurane preconditioning (IsoPC). In vitro, Ubc9 protein expression was evaluated at 4 and 24 h after reoxygenation. The role of Ubc9 in the neuroprotective effect was assessed in the presence or absence of Ubc9 small interfering RNA (siRNA). In vivo, rats were preconditionally exposed for 1 h to 2 % isoflurane for five consecutive days followed by middle cerebral artery occlusion. Neurobehavioral scores and infarction volume were determined at different times after reperfusion. The role of Ubc9 in ischemic tolerance was evaluated by intracerebroventricular microinjection with the Ubc9 siRNA. We showed that isoflurane preconditioning improved the cell viability of the SH-SY5Y cells that were challenged by oxygen-glucose deprivation. It also reduced brain infarct volumes and improved neurologic outcomes in the focal cerebral ischemic rat. The expression of Ubc9 was upregulated by isoflurane preconditioning. Knockdown of Ubc9 significantly attenuated the isoflurane preconditioning-induced neuroprotective effects. Isoflurane preconditioning-induced neuroprotection against ischemic injuries is mediated by Ubc9. These results suggest a novel mechanism for isoflurane preconditioning-induced tolerance to cerebral ischemia.
format Online
Article
Text
id pubmed-4435903
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-44359032015-05-22 The Role of SUMO-Conjugating Enzyme Ubc9 in the Neuroprotection of Isoflurane Preconditioning Against Ischemic Neuronal Injury Tong, Li Wu, Zhixin Ran, Mingzi Chen, Yu Yang, Lujia Zhang, Haopeng Zhang, Lina Dong, Hailong Xiong, Lize Mol Neurobiol Article Preconditioning with volatile anesthetics can create an ischemia tolerance against cerebral ischemia-reperfusion injury. We investigated whether ubiquitin conjugase 9 (Ubc9), the E2 conjugase for SUMOylation, is associated with neuroprotection induced by isoflurane preconditioning (IsoPC). In vitro, Ubc9 protein expression was evaluated at 4 and 24 h after reoxygenation. The role of Ubc9 in the neuroprotective effect was assessed in the presence or absence of Ubc9 small interfering RNA (siRNA). In vivo, rats were preconditionally exposed for 1 h to 2 % isoflurane for five consecutive days followed by middle cerebral artery occlusion. Neurobehavioral scores and infarction volume were determined at different times after reperfusion. The role of Ubc9 in ischemic tolerance was evaluated by intracerebroventricular microinjection with the Ubc9 siRNA. We showed that isoflurane preconditioning improved the cell viability of the SH-SY5Y cells that were challenged by oxygen-glucose deprivation. It also reduced brain infarct volumes and improved neurologic outcomes in the focal cerebral ischemic rat. The expression of Ubc9 was upregulated by isoflurane preconditioning. Knockdown of Ubc9 significantly attenuated the isoflurane preconditioning-induced neuroprotective effects. Isoflurane preconditioning-induced neuroprotection against ischemic injuries is mediated by Ubc9. These results suggest a novel mechanism for isoflurane preconditioning-induced tolerance to cerebral ischemia. Springer US 2014-06-25 2015 /pmc/articles/PMC4435903/ /pubmed/24961570 http://dx.doi.org/10.1007/s12035-014-8797-3 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Tong, Li
Wu, Zhixin
Ran, Mingzi
Chen, Yu
Yang, Lujia
Zhang, Haopeng
Zhang, Lina
Dong, Hailong
Xiong, Lize
The Role of SUMO-Conjugating Enzyme Ubc9 in the Neuroprotection of Isoflurane Preconditioning Against Ischemic Neuronal Injury
title The Role of SUMO-Conjugating Enzyme Ubc9 in the Neuroprotection of Isoflurane Preconditioning Against Ischemic Neuronal Injury
title_full The Role of SUMO-Conjugating Enzyme Ubc9 in the Neuroprotection of Isoflurane Preconditioning Against Ischemic Neuronal Injury
title_fullStr The Role of SUMO-Conjugating Enzyme Ubc9 in the Neuroprotection of Isoflurane Preconditioning Against Ischemic Neuronal Injury
title_full_unstemmed The Role of SUMO-Conjugating Enzyme Ubc9 in the Neuroprotection of Isoflurane Preconditioning Against Ischemic Neuronal Injury
title_short The Role of SUMO-Conjugating Enzyme Ubc9 in the Neuroprotection of Isoflurane Preconditioning Against Ischemic Neuronal Injury
title_sort role of sumo-conjugating enzyme ubc9 in the neuroprotection of isoflurane preconditioning against ischemic neuronal injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4435903/
https://www.ncbi.nlm.nih.gov/pubmed/24961570
http://dx.doi.org/10.1007/s12035-014-8797-3
work_keys_str_mv AT tongli theroleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT wuzhixin theroleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT ranmingzi theroleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT chenyu theroleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT yanglujia theroleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT zhanghaopeng theroleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT zhanglina theroleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT donghailong theroleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT xionglize theroleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT tongli roleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT wuzhixin roleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT ranmingzi roleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT chenyu roleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT yanglujia roleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT zhanghaopeng roleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT zhanglina roleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT donghailong roleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury
AT xionglize roleofsumoconjugatingenzymeubc9intheneuroprotectionofisofluranepreconditioningagainstischemicneuronalinjury