Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments

In the era of synthetic biology, techniques for rapidly constructing a designer long DNA from short DNA fragments are desired. To realize this, we attempted to establish a method for one-step DNA assembly of unprecedentedly large numbers of fragments. The basic technology is the Ordered Gene Assembl...

Descripción completa

Detalles Bibliográficos
Autores principales: Tsuge, Kenji, Sato, Yukari, Kobayashi, Yuka, Gondo, Maiko, Hasebe, Masako, Togashi, Takashi, Tomita, Masaru, Itaya, Mitsuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4438487/
https://www.ncbi.nlm.nih.gov/pubmed/25990947
http://dx.doi.org/10.1038/srep10655

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4438487/
https://www.ncbi.nlm.nih.gov/pubmed/25990947
http://dx.doi.org/10.1038/srep10655

Ejemplares similares