Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes

BACKGROUND: Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing permits the rapid production of genetically engineered mice. To make the most of this innovative technology, a streamlined procedure is needed for the robust con...

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Detalles Bibliográficos
Autores principales: Nakagawa, Yoshiko, Sakuma, Tetsushi, Sakamoto, Takuya, Ohmuraya, Masaki, Nakagata, Naomi, Yamamoto, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440308/
https://www.ncbi.nlm.nih.gov/pubmed/25997509
http://dx.doi.org/10.1186/s12896-015-0144-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440308/
https://www.ncbi.nlm.nih.gov/pubmed/25997509
http://dx.doi.org/10.1186/s12896-015-0144-x

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