Cargando…

Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes

BACKGROUND: Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing permits the rapid production of genetically engineered mice. To make the most of this innovative technology, a streamlined procedure is needed for the robust con...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nakagawa, Yoshiko, Sakuma, Tetsushi, Sakamoto, Takuya, Ohmuraya, Masaki, Nakagata, Naomi, Yamamoto, Takashi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440308/ https://www.ncbi.nlm.nih.gov/pubmed/25997509 http://dx.doi.org/10.1186/s12896-015-0144-x

Ejemplares similares

Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice
por: Nakagawa, Yoshiko, et al.
Publicado: (2017)

Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing
por: Nakagawa, Yoshiko, et al.
Publicado: (2014)

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice
por: Nakagawa, Yoshiko, et al.
Publicado: (2016)

CRISPR/Cas9 microinjection in oocytes disables pancreas development in sheep
por: Vilarino, Marcela, et al.
Publicado: (2017)

Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering
por: Horii, Takuro, et al.
Publicado: (2014)

Screening Methods to Identify TALEN-Mediated Knockout Mice
por: Nakagawa, Yoshiko, et al.
Publicado: (2014)

Author Correction: CRISPR/Cas9 microinjection in oocytes disables pancreas development in sheep
por: Vilarino, Marcela, et al.
Publicado: (2020)

Slow oocyte freezing and thawing in couples with no sperm or an insufficient number of sperm on the day of in vitro fertilization
por: Virant-Klun, Irma, et al.
Publicado: (2011)

Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation
por: Nakagawa, Yoshiko, et al.
Publicado: (2018)

Microinjection of Xenopus Laevis Oocytes
por: Cohen, Sarah, et al.
Publicado: (2009)

Nomogram to predict pregnancy outcomes of emergency oocyte freeze-thaw cycles
por: Wang, Yang, et al.
Publicado: (2021)

Optimization of sand fly embryo microinjection for gene editing by CRISPR/Cas9
por: Martin-Martin, Ines, et al.
Publicado: (2018)

Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote
por: Raveux, Aurélien, et al.
Publicado: (2017)

N-acetyl cysteine restores the fertility of vitrified–warmed mouse oocytes derived through ultrasuperovulation
por: Mukunoki, Ayumi, et al.
Publicado: (2019)

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment
por: Stein, Paula, et al.
Publicado: (2011)

Microinjection induces changes in the transcriptome of bovine oocytes
por: Tan, Minjie, et al.
Publicado: (2020)

Regenerating islet-derived protein (Reg)3β plays a crucial role in attenuation of ileitis and colitis in mice
por: Shindo, Ryodai, et al.
Publicado: (2020)

Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos
por: TANIHARA, Fuminori, et al.
Publicado: (2019)

Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins
Publicado: (1975)

Microinjection Method for Analyzing Zebrafish Early Stage Oocytes
por: Kobayashi, Manami, et al.
Publicado: (2021)

Knockout of a highly GC‐rich gene in Burkholderia pyrrocinia by recombineering with freeze‐thawing transformation
por: Chen, Feifei, et al.
Publicado: (2021)

Heat transfer with freezing and thawing
por: Lunardini, VJ
Publicado: (1991)

Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes
por: Crispo, M., et al.
Publicado: (2015)

Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
por: Le, Quynh Anh, et al.
Publicado: (2021)

CRISPR-Cas9-induced gene knockout in zebrafish
por: Medishetti, Raghavender, et al.
Publicado: (2022)

A hypomorphic mutation in the mouse Csn1s1 gene generated by CRISPR/Cas9 pronuclear microinjection
por: Smirnov, A.V., et al.
Publicado: (2021)

Synchronization of the ovulation and copulation timings increased the number of in vivo fertilized oocytes in superovulated female mice
por: Nakao, Satohiro, et al.
Publicado: (2023)

Effect of diluent type, cryoprotectant concentration, storage method and freeze/thaw rates on the post-thaw quality and fertility of cryopreserved alpaca spermatozoa
por: Stuart, C. C., et al.
Publicado: (2019)

Social freezing of oocytes: a means to take control of your fertility
por: Wennberg, Anna-Lena
Publicado: (2020)

Production of Sry knockout mouse using TALEN via oocyte injection
por: Kato, Tomoko, et al.
Publicado: (2013)

Freezing and thawing magnetic droplet solitons
por: Ahlberg, Martina, et al.
Publicado: (2022)

Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9
por: Nakade, Shota, et al.
Publicado: (2014)

Export of mRNA from microinjected nuclei of Xenopus laevis oocytes
Publicado: (1992)

Fabrication on the microscale: a two-photon polymerized device for oocyte microinjection
por: Yagoub, Suliman H., et al.
Publicado: (2022)

Controlled delivery of β-globin-targeting TALENs and CRISPR/Cas9 into mammalian cells for genome editing using microinjection
por: Cottle, Renee N., et al.
Publicado: (2015)

HT-B and S-RNase CRISPR-Cas9 double knockouts show enhanced self-fertility in diploid Solanum tuberosum
por: Lee, Sarah, et al.
Publicado: (2023)

Developmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa
por: Scholkamy, T. H., et al.
Publicado: (2016)

JACKS: joint analysis of CRISPR/Cas9 knockout screens
por: Allen, Felicity, et al.
Publicado: (2019)

Prolonged exposure to hyaluronidase decreases the fertilization and development rates of fresh and cryopreserved mouse oocytes
por: ISHIZUKA, Yuta, et al.
Publicado: (2014)

Freeze–thaw Caenorhabditis elegans freeze–thaw stress response is regulated by the insulin/IGF-1 receptor daf-2
por: Hu, Jian-Ping, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_8l8bcpu8hb3jc3ga09bf4fpm7b