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Time-Domain Microfluidic Fluorescence Lifetime Flow Cytometry for High-Throughput Förster Resonance Energy Transfer Screening

Sensing ion or ligand concentrations, physico-chemical conditions, and molecular dimerization or conformation change is possible by assays involving fluorescent lifetime imaging. The inherent low throughput of imaging impedes rigorous statistical data analysis on large cell numbers. We address this...

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Detalles Bibliográficos
Autores principales: Nedbal, Jakub, Visitkul, Viput, Ortiz-Zapater, Elena, Weitsman, Gregory, Chana, Prabhjoat, Matthews, Daniel R, Ng, Tony, Ameer-Beg, Simon M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440390/
https://www.ncbi.nlm.nih.gov/pubmed/25523156
http://dx.doi.org/10.1002/cyto.a.22616