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Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth
We examined the thymoquinone induced inhibition of purified F(1) or membrane bound F(1)F(O) E. coli ATP synthase. Both purified F(1) and membrane bound F(1)F(O) were completely inhibited by thymoquinone with no residual ATPase activity. The process of inhibition was fully reversible and identical in...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440651/ https://www.ncbi.nlm.nih.gov/pubmed/25996607 http://dx.doi.org/10.1371/journal.pone.0127802 |
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author | Ahmad, Zulfiqar Laughlin, Thomas F. Kady, Ismail O. |
author_facet | Ahmad, Zulfiqar Laughlin, Thomas F. Kady, Ismail O. |
author_sort | Ahmad, Zulfiqar |
collection | PubMed |
description | We examined the thymoquinone induced inhibition of purified F(1) or membrane bound F(1)F(O) E. coli ATP synthase. Both purified F(1) and membrane bound F(1)F(O) were completely inhibited by thymoquinone with no residual ATPase activity. The process of inhibition was fully reversible and identical in both membrane bound F(1)F(o) and purified F(1) preparations. Moreover, thymoquinone induced inhibition of ATP synthase expressing wild-type E. coli cell growth and non-inhibition of ATPase gene deleted null control cells demonstrates that ATP synthase is a molecular target for thymoquinone. This also links the beneficial dietary based antimicrobial and anticancer effects of thymoquinone to its inhibitory action on ATP synthase. |
format | Online Article Text |
id | pubmed-4440651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44406512015-05-29 Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth Ahmad, Zulfiqar Laughlin, Thomas F. Kady, Ismail O. PLoS One Research Article We examined the thymoquinone induced inhibition of purified F(1) or membrane bound F(1)F(O) E. coli ATP synthase. Both purified F(1) and membrane bound F(1)F(O) were completely inhibited by thymoquinone with no residual ATPase activity. The process of inhibition was fully reversible and identical in both membrane bound F(1)F(o) and purified F(1) preparations. Moreover, thymoquinone induced inhibition of ATP synthase expressing wild-type E. coli cell growth and non-inhibition of ATPase gene deleted null control cells demonstrates that ATP synthase is a molecular target for thymoquinone. This also links the beneficial dietary based antimicrobial and anticancer effects of thymoquinone to its inhibitory action on ATP synthase. Public Library of Science 2015-05-21 /pmc/articles/PMC4440651/ /pubmed/25996607 http://dx.doi.org/10.1371/journal.pone.0127802 Text en © 2015 Ahmad et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ahmad, Zulfiqar Laughlin, Thomas F. Kady, Ismail O. Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth |
title | Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth |
title_full | Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth |
title_fullStr | Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth |
title_full_unstemmed | Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth |
title_short | Thymoquinone Inhibits Escherichia coli ATP Synthase and Cell Growth |
title_sort | thymoquinone inhibits escherichia coli atp synthase and cell growth |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440651/ https://www.ncbi.nlm.nih.gov/pubmed/25996607 http://dx.doi.org/10.1371/journal.pone.0127802 |
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