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N-Acetyl-D-Glucosamine Kinase Is a Component of Nuclear Speckles and Paraspeckles

Protein O-GlcNAcylation, dictated by cellular UDP-N-acetylglucosamine (UDP-GlcNAc) levels, plays a crucial role in posttranslational modifications. The enzyme GlcNAc kinase (NAGK, E.C. 2.7.1.59) catalyzes the formation of GlcNAc-6-phosphate, which is a major substrate for the biosynthesis of UDP-Glc...

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Autores principales: Sharif, Syeda Ridita, Lee, HyunSook, Islam, Md. Ariful, Seog, Dae-Hyun, Moon, Il Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443281/
https://www.ncbi.nlm.nih.gov/pubmed/25921606
http://dx.doi.org/10.14348/molcells.2015.2242
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author Sharif, Syeda Ridita
Lee, HyunSook
Islam, Md. Ariful
Seog, Dae-Hyun
Moon, Il Soo
author_facet Sharif, Syeda Ridita
Lee, HyunSook
Islam, Md. Ariful
Seog, Dae-Hyun
Moon, Il Soo
author_sort Sharif, Syeda Ridita
collection PubMed
description Protein O-GlcNAcylation, dictated by cellular UDP-N-acetylglucosamine (UDP-GlcNAc) levels, plays a crucial role in posttranslational modifications. The enzyme GlcNAc kinase (NAGK, E.C. 2.7.1.59) catalyzes the formation of GlcNAc-6-phosphate, which is a major substrate for the biosynthesis of UDP-GlcNAc. Recent studies have revealed the expression of NAGK in different types of cells especially in neuronal dendrites. Here, by immunocytochemistry (ICC) and immunonucleochemistry (INC) of cultured rat hippocampal neurons, HEK293T and GT1-7 cells, we have showed that NAGK immuno-reactive punctae being present in the nucleoplasm colocalized with small nuclear ribonucleoprotein-associated protein N (snRNPN) and p54NRB, which are speckle and paraspeckle markers, respectively. Furthermore, NAGK IR cluster was also found to be colocalized with GTF2H5 (general transcription factor IIH, polypeptide 5) immuno reactive punctae. In addition, relative localization to the ring of nuclear lamin matrix and to GlcNAc, which is highly enriched in nuclear pore complexes, showed that NAGK surrounds the nucleus at the cytoplasmic face of the nuclear outer membrane. By in situ proximity ligation assay (PLA) we confirmed the colocalization of NAGK with snRNPN in the nucleus and in dendrites, while we also verified the interactions of NAGK with p54NRB, and with GTF2H5 in the nucleus. These associations between NAGK with speckle, paraspeckle and general transcription factor suggest its regulatory roles in gene expression.
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spelling pubmed-44432812015-06-01 N-Acetyl-D-Glucosamine Kinase Is a Component of Nuclear Speckles and Paraspeckles Sharif, Syeda Ridita Lee, HyunSook Islam, Md. Ariful Seog, Dae-Hyun Moon, Il Soo Mol Cells Article Protein O-GlcNAcylation, dictated by cellular UDP-N-acetylglucosamine (UDP-GlcNAc) levels, plays a crucial role in posttranslational modifications. The enzyme GlcNAc kinase (NAGK, E.C. 2.7.1.59) catalyzes the formation of GlcNAc-6-phosphate, which is a major substrate for the biosynthesis of UDP-GlcNAc. Recent studies have revealed the expression of NAGK in different types of cells especially in neuronal dendrites. Here, by immunocytochemistry (ICC) and immunonucleochemistry (INC) of cultured rat hippocampal neurons, HEK293T and GT1-7 cells, we have showed that NAGK immuno-reactive punctae being present in the nucleoplasm colocalized with small nuclear ribonucleoprotein-associated protein N (snRNPN) and p54NRB, which are speckle and paraspeckle markers, respectively. Furthermore, NAGK IR cluster was also found to be colocalized with GTF2H5 (general transcription factor IIH, polypeptide 5) immuno reactive punctae. In addition, relative localization to the ring of nuclear lamin matrix and to GlcNAc, which is highly enriched in nuclear pore complexes, showed that NAGK surrounds the nucleus at the cytoplasmic face of the nuclear outer membrane. By in situ proximity ligation assay (PLA) we confirmed the colocalization of NAGK with snRNPN in the nucleus and in dendrites, while we also verified the interactions of NAGK with p54NRB, and with GTF2H5 in the nucleus. These associations between NAGK with speckle, paraspeckle and general transcription factor suggest its regulatory roles in gene expression. Korean Society for Molecular and Cellular Biology 2015-05-31 2015-04-28 /pmc/articles/PMC4443281/ /pubmed/25921606 http://dx.doi.org/10.14348/molcells.2015.2242 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Sharif, Syeda Ridita
Lee, HyunSook
Islam, Md. Ariful
Seog, Dae-Hyun
Moon, Il Soo
N-Acetyl-D-Glucosamine Kinase Is a Component of Nuclear Speckles and Paraspeckles
title N-Acetyl-D-Glucosamine Kinase Is a Component of Nuclear Speckles and Paraspeckles
title_full N-Acetyl-D-Glucosamine Kinase Is a Component of Nuclear Speckles and Paraspeckles
title_fullStr N-Acetyl-D-Glucosamine Kinase Is a Component of Nuclear Speckles and Paraspeckles
title_full_unstemmed N-Acetyl-D-Glucosamine Kinase Is a Component of Nuclear Speckles and Paraspeckles
title_short N-Acetyl-D-Glucosamine Kinase Is a Component of Nuclear Speckles and Paraspeckles
title_sort n-acetyl-d-glucosamine kinase is a component of nuclear speckles and paraspeckles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443281/
https://www.ncbi.nlm.nih.gov/pubmed/25921606
http://dx.doi.org/10.14348/molcells.2015.2242
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