An overhang-based DNA block shuffling method for creating a customized random library

We present an overhang-based DNA block shuffling method to create a customized random DNA library with flexible sequence design and length. Our method enables the efficient and seamless assembly of short DNA blocks with dinucleotide overhangs through a simple ligation process. Next generation sequen...

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Detalles Bibliográficos
Autores principales: Fujishima, Kosuke, Venter, Chris, Wang, Kendrick, Ferreira, Raphael, Rothschild, Lynn J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443763/
https://www.ncbi.nlm.nih.gov/pubmed/26010273
http://dx.doi.org/10.1038/srep09740

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443763/
https://www.ncbi.nlm.nih.gov/pubmed/26010273
http://dx.doi.org/10.1038/srep09740

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