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An overhang-based DNA block shuffling method for creating a customized random library
We present an overhang-based DNA block shuffling method to create a customized random DNA library with flexible sequence design and length. Our method enables the efficient and seamless assembly of short DNA blocks with dinucleotide overhangs through a simple ligation process. Next generation sequen...
Autores principales: | Fujishima, Kosuke, Venter, Chris, Wang, Kendrick, Ferreira, Raphael, Rothschild, Lynn J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4443763/ https://www.ncbi.nlm.nih.gov/pubmed/26010273 http://dx.doi.org/10.1038/srep09740 |
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