Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish

The type II clustered regularly interspaced short palindromic repeats (CRISPR) associated with Cas9 endonuclease (CRISPR/Cas9) has become a powerful genetic tool for understanding the function of a gene of interest. In zebrafish, the injection of Cas9 mRNA and guide-RNA (gRNA), which are prepared us...

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Detalles Bibliográficos
Autores principales: Kotani, Hirohito, Taimatsu, Kiyohito, Ohga, Rie, Ota, Satoshi, Kawahara, Atsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444095/
https://www.ncbi.nlm.nih.gov/pubmed/26010089
http://dx.doi.org/10.1371/journal.pone.0128319

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444095/
https://www.ncbi.nlm.nih.gov/pubmed/26010089
http://dx.doi.org/10.1371/journal.pone.0128319

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