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Ligation-anchored PCR unveils immune repertoire of TCR-beta from whole blood
BACKGROUND: As one of the genetic mechanisms for adaptive immunity, V(D)J recombination generates an enormous repertoire of T-cell receptors (TCRs). With the development of high-throughput sequencing techniques, systematic exploration of V(D)J recombination becomes possible. Multiplex PCR has been p...
Autores principales: | Gao, Fan, Wang, Kai |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446965/ https://www.ncbi.nlm.nih.gov/pubmed/26018870 http://dx.doi.org/10.1186/s12896-015-0153-9 |
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