High-throughput monitoring of integration site clonality in preclinical and clinical gene therapy studies

Gene transfer to hematopoietic stem cells with integrating vectors not only allows sustained correction of monogenic diseases but also tracking of individual clones in vivo. Quantitative real-time PCR (qPCR) has been shown to be an accurate method to quantify individual stem cell clones, yet due to...

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Detalles Bibliográficos
Autores principales: Giordano, Frank A, Appelt, Jens-Uwe, Link, Barbara, Gerdes, Sebastian, Lehrer, Christina, Scholz, Simone, Paruzynski, Anna, Roeder, Ingo, Wenz, Frederik, Glimm, Hanno, von Kalle, Christof, Grez, Manuel, Schmidt, Manfred, Laufs, Stephanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449016/
https://www.ncbi.nlm.nih.gov/pubmed/26052530
http://dx.doi.org/10.1038/mtm.2014.61

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449016/
https://www.ncbi.nlm.nih.gov/pubmed/26052530
http://dx.doi.org/10.1038/mtm.2014.61

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