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Optimization of Fermentation Conditions for Recombinant Human Interferon Beta Production by Escherichia coli Using the Response Surface Methodology

BACKGROUND: The periplasmic overexpression of recombinant human interferon beta (rhIFN-β)-1b using a synthetic gene in Escherichia coli BL21 (DE3) was optimized in shake flasks using Response Surface Methodology (RSM) based on the Box-Behnken Design (BBD). OBJECTIVES: This study aimed to predict and...

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Detalles Bibliográficos
Autores principales:	Morowvat, Mohammad Hossein, Babaeipour, Valiollah, Rajabi Memari, Hamid, Vahidi, Hossein
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Kowsar 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449858/ https://www.ncbi.nlm.nih.gov/pubmed/26034535 http://dx.doi.org/10.5812/jjm.8(4)2015.16236

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