Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats

The purpose of this study was to examine the ultrastructural changes of surface cartilage collagen fibers, which differ by region and the length of the experimental period in an immobilization model of rat. Male Wistar rats were randomly divided into histological or macroscopic and ultrastructural a...

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Autores principales: Nagai, Momoko, Aoyama, Tomoki, Ito, Akira, Tajino, Junichi, Iijima, Hirotaka, Yamaguchi, Shoki, Zhang, Xiangkai, Kuroki, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4450945/
https://www.ncbi.nlm.nih.gov/pubmed/25939458
http://dx.doi.org/10.1111/joa.12290
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author Nagai, Momoko
Aoyama, Tomoki
Ito, Akira
Tajino, Junichi
Iijima, Hirotaka
Yamaguchi, Shoki
Zhang, Xiangkai
Kuroki, Hiroshi
author_facet Nagai, Momoko
Aoyama, Tomoki
Ito, Akira
Tajino, Junichi
Iijima, Hirotaka
Yamaguchi, Shoki
Zhang, Xiangkai
Kuroki, Hiroshi
author_sort Nagai, Momoko
collection PubMed
description The purpose of this study was to examine the ultrastructural changes of surface cartilage collagen fibers, which differ by region and the length of the experimental period in an immobilization model of rat. Male Wistar rats were randomly divided into histological or macroscopic and ultrastructural assessment groups. The left knees of all the animals were surgically immobilized by external fixation for 1, 2, 4, 8 or 16 weeks (n = 5/time point). Sagittal histological sections of the medial mid-condylar region of the knee were obtained and assessed in four specific regions (contact and peripheral regions of the femur and tibia) and two zones (superficial and deep). To semi-quantify the staining intensity of the collagen fibers in the cartilage, picrosirius red staining was used. The cartilage surface changes of all the assessed regions were investigated by scanning electron microscopy (SEM). From histological and SEM observations, the fibrillation and irregular changes of the cartilage surface were more severe in the peripheral region than in the contact region. Interestingly, at 16 weeks post-immobilization, we observed non-fibrous structures at both the contact and peripheral regions. The collagen fiber staining intensity decreased in the contact region compared with the peripheral region. In conclusion, the alteration of surface collagen fiber ultrastructure and collagen staining intensity differed by the specific cartilage regions after immobilization. These results demonstrate that the progressive degeneration of cartilage is region specific, and depends on the length of the immobilization period.
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spelling pubmed-44509452016-10-14 Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats Nagai, Momoko Aoyama, Tomoki Ito, Akira Tajino, Junichi Iijima, Hirotaka Yamaguchi, Shoki Zhang, Xiangkai Kuroki, Hiroshi J Anat Original Articles The purpose of this study was to examine the ultrastructural changes of surface cartilage collagen fibers, which differ by region and the length of the experimental period in an immobilization model of rat. Male Wistar rats were randomly divided into histological or macroscopic and ultrastructural assessment groups. The left knees of all the animals were surgically immobilized by external fixation for 1, 2, 4, 8 or 16 weeks (n = 5/time point). Sagittal histological sections of the medial mid-condylar region of the knee were obtained and assessed in four specific regions (contact and peripheral regions of the femur and tibia) and two zones (superficial and deep). To semi-quantify the staining intensity of the collagen fibers in the cartilage, picrosirius red staining was used. The cartilage surface changes of all the assessed regions were investigated by scanning electron microscopy (SEM). From histological and SEM observations, the fibrillation and irregular changes of the cartilage surface were more severe in the peripheral region than in the contact region. Interestingly, at 16 weeks post-immobilization, we observed non-fibrous structures at both the contact and peripheral regions. The collagen fiber staining intensity decreased in the contact region compared with the peripheral region. In conclusion, the alteration of surface collagen fiber ultrastructure and collagen staining intensity differed by the specific cartilage regions after immobilization. These results demonstrate that the progressive degeneration of cartilage is region specific, and depends on the length of the immobilization period. BlackWell Publishing Ltd 2015-05 2015-05-04 /pmc/articles/PMC4450945/ /pubmed/25939458 http://dx.doi.org/10.1111/joa.12290 Text en © 2015 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Nagai, Momoko
Aoyama, Tomoki
Ito, Akira
Tajino, Junichi
Iijima, Hirotaka
Yamaguchi, Shoki
Zhang, Xiangkai
Kuroki, Hiroshi
Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats
title Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats
title_full Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats
title_fullStr Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats
title_full_unstemmed Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats
title_short Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats
title_sort alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4450945/
https://www.ncbi.nlm.nih.gov/pubmed/25939458
http://dx.doi.org/10.1111/joa.12290
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