A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis

BACKGROUND: Seamless ligation cloning extract (SLiCE) is a simple and efficient method for DNA assembly that uses cell extracts from the Escherichia coli PPY strain, which expresses the components of the λ prophage Red/ET recombination system. This method facilitates restriction endonuclease cleavag...

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Detalles Bibliográficos
Autor principal: Motohashi, Ken
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4453199/
https://www.ncbi.nlm.nih.gov/pubmed/26037246
http://dx.doi.org/10.1186/s12896-015-0162-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4453199/
https://www.ncbi.nlm.nih.gov/pubmed/26037246
http://dx.doi.org/10.1186/s12896-015-0162-8

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