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Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2

Our understanding of the genus Natrinema is presently limited due to the lack of available genetic tools. Auxotrophic markers have been widely used to construct genetic systems in bacteria and eukaryotes and in some archaeal species. Here, we isolated four auxotrophic mutants of Natrinema sp. J7-2,...

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Detalles Bibliográficos
Autores principales: Lv, Jie, Wang, Shuai, Wang, Yuchen, Huang, Yuping, Chen, Xiangdong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454726/
https://www.ncbi.nlm.nih.gov/pubmed/26089742
http://dx.doi.org/10.1155/2015/483194