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Chitosan/siCkip-1 biofunctionalized titanium implant for improved osseointegration in the osteoporotic condition
Biofunctionalization with siRNA targeting the key negative modulators of bone turnover involved in the molecular mechanism of osteoporosis, such as casein kinase-2 interacting protein-1 (Ckip-1), may lead to enhanced Ti osseointegration in the osteoporotic condition. In this study, even siRNA loadin...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455222/ https://www.ncbi.nlm.nih.gov/pubmed/26040545 http://dx.doi.org/10.1038/srep10860 |
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author | Zhang, Li Wu, Kaimin Song, Wen Xu, Haiyan An, Ran Zhao, Lingzhou Liu, Bin Zhang, Yumei |
author_facet | Zhang, Li Wu, Kaimin Song, Wen Xu, Haiyan An, Ran Zhao, Lingzhou Liu, Bin Zhang, Yumei |
author_sort | Zhang, Li |
collection | PubMed |
description | Biofunctionalization with siRNA targeting the key negative modulators of bone turnover involved in the molecular mechanism of osteoporosis, such as casein kinase-2 interacting protein-1 (Ckip-1), may lead to enhanced Ti osseointegration in the osteoporotic condition. In this study, even siRNA loading was accomplished by the thermal alkali (TA) treatment to make the Ti ultrahydrophilic and negatively charged to facilitate the physical adsorption of the positively charged CS/siR complex, designated as TA-CS/siR. The intracellular uptake of the CS/siR complex and the gene knockdown efficiency were assessed with bone marrow mesenchymal stem cells (MSCs) as well as the green fluorescent protein (GFP) expressing H1299 cells. In vitro osteogenic activity of TA-CS/siCkip-1 targeting Ckip-1 was assessed with MSCs. In vivo osseointegration of TA-CS/siCkip-1 was assessed in the osteoporotic rat model. TA-CS/siR showed excellent siRNA delivery efficiency and gene silencing effect. TA-CS/siCkip-1 significantly improved the in vitro osteogenic differentiation of MSCs in terms of the enhanced alkaline phosphatase and collagen product and extracellular matrix mineralization, and led to dramatically enhanced in vivo osseointegration in the osteoporostic rat model, showing promising clinical potential for the osteoporotic condition application. TA-CS/siR may constitute a general approach for developing the advanced Ti implants targeting specific molecular mechanism. |
format | Online Article Text |
id | pubmed-4455222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-44552222015-06-10 Chitosan/siCkip-1 biofunctionalized titanium implant for improved osseointegration in the osteoporotic condition Zhang, Li Wu, Kaimin Song, Wen Xu, Haiyan An, Ran Zhao, Lingzhou Liu, Bin Zhang, Yumei Sci Rep Article Biofunctionalization with siRNA targeting the key negative modulators of bone turnover involved in the molecular mechanism of osteoporosis, such as casein kinase-2 interacting protein-1 (Ckip-1), may lead to enhanced Ti osseointegration in the osteoporotic condition. In this study, even siRNA loading was accomplished by the thermal alkali (TA) treatment to make the Ti ultrahydrophilic and negatively charged to facilitate the physical adsorption of the positively charged CS/siR complex, designated as TA-CS/siR. The intracellular uptake of the CS/siR complex and the gene knockdown efficiency were assessed with bone marrow mesenchymal stem cells (MSCs) as well as the green fluorescent protein (GFP) expressing H1299 cells. In vitro osteogenic activity of TA-CS/siCkip-1 targeting Ckip-1 was assessed with MSCs. In vivo osseointegration of TA-CS/siCkip-1 was assessed in the osteoporotic rat model. TA-CS/siR showed excellent siRNA delivery efficiency and gene silencing effect. TA-CS/siCkip-1 significantly improved the in vitro osteogenic differentiation of MSCs in terms of the enhanced alkaline phosphatase and collagen product and extracellular matrix mineralization, and led to dramatically enhanced in vivo osseointegration in the osteoporostic rat model, showing promising clinical potential for the osteoporotic condition application. TA-CS/siR may constitute a general approach for developing the advanced Ti implants targeting specific molecular mechanism. Nature Publishing Group 2015-06-04 /pmc/articles/PMC4455222/ /pubmed/26040545 http://dx.doi.org/10.1038/srep10860 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhang, Li Wu, Kaimin Song, Wen Xu, Haiyan An, Ran Zhao, Lingzhou Liu, Bin Zhang, Yumei Chitosan/siCkip-1 biofunctionalized titanium implant for improved osseointegration in the osteoporotic condition |
title | Chitosan/siCkip-1 biofunctionalized titanium implant for improved osseointegration in the osteoporotic condition |
title_full | Chitosan/siCkip-1 biofunctionalized titanium implant for improved osseointegration in the osteoporotic condition |
title_fullStr | Chitosan/siCkip-1 biofunctionalized titanium implant for improved osseointegration in the osteoporotic condition |
title_full_unstemmed | Chitosan/siCkip-1 biofunctionalized titanium implant for improved osseointegration in the osteoporotic condition |
title_short | Chitosan/siCkip-1 biofunctionalized titanium implant for improved osseointegration in the osteoporotic condition |
title_sort | chitosan/sickip-1 biofunctionalized titanium implant for improved osseointegration in the osteoporotic condition |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455222/ https://www.ncbi.nlm.nih.gov/pubmed/26040545 http://dx.doi.org/10.1038/srep10860 |
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