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Aluminium-phthalocyanine chloride nanoemulsions for anticancer photodynamic therapy: Development and in vitro activity against monolayers and spheroids of human mammary adenocarcinoma MCF-7 cells

BACKGROUND: Photodynamic therapy (PDT) combines light, molecular oxygen and a photosensitizer to induce oxidative stress in target cells. Certain hydrophobic photosensitizers, such as aluminium-phthalocyanine chloride (AlPc), have significant potential for antitumor PDT applications. However, hydrop...

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Autores principales: Muehlmann, Luis Alexandre, Rodrigues, Mosar Corrêa, Longo, João Paulo Figueiró, Garcia, Mônica Pereira, Py-Daniel, Karen Rapp, Veloso, Aline Bessa, de Souza, Paulo Eduardo Narciso, da Silva, Sebastião William, Azevedo, Ricardo Bentes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455699/
https://www.ncbi.nlm.nih.gov/pubmed/25966866
http://dx.doi.org/10.1186/s12951-015-0095-3
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author Muehlmann, Luis Alexandre
Rodrigues, Mosar Corrêa
Longo, João Paulo Figueiró
Garcia, Mônica Pereira
Py-Daniel, Karen Rapp
Veloso, Aline Bessa
de Souza, Paulo Eduardo Narciso
da Silva, Sebastião William
Azevedo, Ricardo Bentes
author_facet Muehlmann, Luis Alexandre
Rodrigues, Mosar Corrêa
Longo, João Paulo Figueiró
Garcia, Mônica Pereira
Py-Daniel, Karen Rapp
Veloso, Aline Bessa
de Souza, Paulo Eduardo Narciso
da Silva, Sebastião William
Azevedo, Ricardo Bentes
author_sort Muehlmann, Luis Alexandre
collection PubMed
description BACKGROUND: Photodynamic therapy (PDT) combines light, molecular oxygen and a photosensitizer to induce oxidative stress in target cells. Certain hydrophobic photosensitizers, such as aluminium-phthalocyanine chloride (AlPc), have significant potential for antitumor PDT applications. However, hydrophobic molecules often require drug-delivery systems, such as nanostructures, to improve their pharmacokinetic properties and to prevent aggregation, which has a quenching effect on the photoemission properties in aqueous media. As a result, this work aims to develop and test the efficacy of an AlPc in the form of a nanoemulsion to enable its use in anticancer PDT. RESULTS: The nanoemulsion was developed using castor oil and Cremophor ELP®, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 25 nm was obtained. While free AlPc failed to show significant activity against human breast adenocarcinoma MCF-7 cells in an in vitro PDT assay, the AlPc in the nanoemulsion showed intense photodynamic activity. Photoactivated AlPc exhibited a 50 % cytotoxicity concentration (CC50) of 6.0 nM when applied to MCF-7 cell monolayers and exerted a powerful cytotoxic effect on MCF-7 cell spheroids. CONCLUSION: Through the use of spontaneous emulsification, a stable AlPc nanoemulsion was developed that exhibits strong in vitro photodynamic activity on cancer cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12951-015-0095-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-44556992015-06-05 Aluminium-phthalocyanine chloride nanoemulsions for anticancer photodynamic therapy: Development and in vitro activity against monolayers and spheroids of human mammary adenocarcinoma MCF-7 cells Muehlmann, Luis Alexandre Rodrigues, Mosar Corrêa Longo, João Paulo Figueiró Garcia, Mônica Pereira Py-Daniel, Karen Rapp Veloso, Aline Bessa de Souza, Paulo Eduardo Narciso da Silva, Sebastião William Azevedo, Ricardo Bentes J Nanobiotechnology Research BACKGROUND: Photodynamic therapy (PDT) combines light, molecular oxygen and a photosensitizer to induce oxidative stress in target cells. Certain hydrophobic photosensitizers, such as aluminium-phthalocyanine chloride (AlPc), have significant potential for antitumor PDT applications. However, hydrophobic molecules often require drug-delivery systems, such as nanostructures, to improve their pharmacokinetic properties and to prevent aggregation, which has a quenching effect on the photoemission properties in aqueous media. As a result, this work aims to develop and test the efficacy of an AlPc in the form of a nanoemulsion to enable its use in anticancer PDT. RESULTS: The nanoemulsion was developed using castor oil and Cremophor ELP®, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 25 nm was obtained. While free AlPc failed to show significant activity against human breast adenocarcinoma MCF-7 cells in an in vitro PDT assay, the AlPc in the nanoemulsion showed intense photodynamic activity. Photoactivated AlPc exhibited a 50 % cytotoxicity concentration (CC50) of 6.0 nM when applied to MCF-7 cell monolayers and exerted a powerful cytotoxic effect on MCF-7 cell spheroids. CONCLUSION: Through the use of spontaneous emulsification, a stable AlPc nanoemulsion was developed that exhibits strong in vitro photodynamic activity on cancer cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12951-015-0095-3) contains supplementary material, which is available to authorized users. BioMed Central 2015-05-13 /pmc/articles/PMC4455699/ /pubmed/25966866 http://dx.doi.org/10.1186/s12951-015-0095-3 Text en © Muehlmann et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Muehlmann, Luis Alexandre
Rodrigues, Mosar Corrêa
Longo, João Paulo Figueiró
Garcia, Mônica Pereira
Py-Daniel, Karen Rapp
Veloso, Aline Bessa
de Souza, Paulo Eduardo Narciso
da Silva, Sebastião William
Azevedo, Ricardo Bentes
Aluminium-phthalocyanine chloride nanoemulsions for anticancer photodynamic therapy: Development and in vitro activity against monolayers and spheroids of human mammary adenocarcinoma MCF-7 cells
title Aluminium-phthalocyanine chloride nanoemulsions for anticancer photodynamic therapy: Development and in vitro activity against monolayers and spheroids of human mammary adenocarcinoma MCF-7 cells
title_full Aluminium-phthalocyanine chloride nanoemulsions for anticancer photodynamic therapy: Development and in vitro activity against monolayers and spheroids of human mammary adenocarcinoma MCF-7 cells
title_fullStr Aluminium-phthalocyanine chloride nanoemulsions for anticancer photodynamic therapy: Development and in vitro activity against monolayers and spheroids of human mammary adenocarcinoma MCF-7 cells
title_full_unstemmed Aluminium-phthalocyanine chloride nanoemulsions for anticancer photodynamic therapy: Development and in vitro activity against monolayers and spheroids of human mammary adenocarcinoma MCF-7 cells
title_short Aluminium-phthalocyanine chloride nanoemulsions for anticancer photodynamic therapy: Development and in vitro activity against monolayers and spheroids of human mammary adenocarcinoma MCF-7 cells
title_sort aluminium-phthalocyanine chloride nanoemulsions for anticancer photodynamic therapy: development and in vitro activity against monolayers and spheroids of human mammary adenocarcinoma mcf-7 cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455699/
https://www.ncbi.nlm.nih.gov/pubmed/25966866
http://dx.doi.org/10.1186/s12951-015-0095-3
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