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Phenotypes of trypsin- and collagenase-prepared bovine corneal endothelial cells in the presence of a selective Rho kinase inhibitor, Y-27632

PURPOSE: To optimize isolation of viable bovine corneal endothelial cells (BCECs), we evaluated the effectiveness of various preparation protocols. This entailed comparing the effects of collagenase A and trypsin in the presence and absence of a Rho kinase inhibitor, Y-27632, on proliferation and ti...

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Detalles Bibliográficos
Autores principales: Su, Chien-Chia, Chen, Chun-Wen, Ho, Wei-Ting, Hu, Fung-Rong, Lee, Shwu-Huey, Wang, I-Jong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455892/
https://www.ncbi.nlm.nih.gov/pubmed/26097378