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Quantitative analysis of the Escherichia coli proteome

Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC–MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quan...

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Detalles Bibliográficos
Autores principales: Wiśniewski, Jacek R., Rakus, Dariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459560/
https://www.ncbi.nlm.nih.gov/pubmed/26217677
http://dx.doi.org/10.1016/j.dib.2014.08.004