Quantitative analysis of the Escherichia coli proteome

Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC–MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quan...

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Detalles Bibliográficos
Autores principales: Wiśniewski, Jacek R., Rakus, Dariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459560/
https://www.ncbi.nlm.nih.gov/pubmed/26217677
http://dx.doi.org/10.1016/j.dib.2014.08.004
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author Wiśniewski, Jacek R.
Rakus, Dariusz
author_facet Wiśniewski, Jacek R.
Rakus, Dariusz
author_sort Wiśniewski, Jacek R.
collection PubMed
description Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC–MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quantitation was performed using the ‘Total Protein Approach’. The data are supplied in the article.
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spelling pubmed-44595602015-07-27 Quantitative analysis of the Escherichia coli proteome Wiśniewski, Jacek R. Rakus, Dariusz Data Brief Data Article Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC–MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quantitation was performed using the ‘Total Protein Approach’. The data are supplied in the article. Elsevier 2014-08-22 /pmc/articles/PMC4459560/ /pubmed/26217677 http://dx.doi.org/10.1016/j.dib.2014.08.004 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Data Article
Wiśniewski, Jacek R.
Rakus, Dariusz
Quantitative analysis of the Escherichia coli proteome
title Quantitative analysis of the Escherichia coli proteome
title_full Quantitative analysis of the Escherichia coli proteome
title_fullStr Quantitative analysis of the Escherichia coli proteome
title_full_unstemmed Quantitative analysis of the Escherichia coli proteome
title_short Quantitative analysis of the Escherichia coli proteome
title_sort quantitative analysis of the escherichia coli proteome
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459560/
https://www.ncbi.nlm.nih.gov/pubmed/26217677
http://dx.doi.org/10.1016/j.dib.2014.08.004
work_keys_str_mv AT wisniewskijacekr quantitativeanalysisoftheescherichiacoliproteome
AT rakusdariusz quantitativeanalysisoftheescherichiacoliproteome

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