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An endogenous ‘non-specific’ protein detected by a His-tag antibody is human transcription regulator YY1

Histidine-tags have been used for a wide variety of experiments including protein purification, Western blots, immunoprecipitation and immunohistochemistry. In our previous studies, we have repeatedly detected a ‘non-specific’ endogenous protein of about 60 kD in Western blots of protein lysates fro...

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Detalles Bibliográficos
Autores principales: Mahmood, Niaz, Xie, Jiuyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459774/
https://www.ncbi.nlm.nih.gov/pubmed/26217706
http://dx.doi.org/10.1016/j.dib.2014.12.002