Fast and reliable production, purification and characterization of heat-stable, bifunctional enzyme chimeras

Degradation of complex plant biomass demands a fine-regulated portfolio of glycoside hydrolases. The LE (LguI/Eco81I)-cloning approach was used to produce two enzyme chimeras CB and BC composed of an endoglucanase Cel5A (C) from the extreme thermophilic bacterium Fervidobacterium gondwanense and an...

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Detalles Bibliográficos
Autores principales: Neddersen, Mara, Elleuche, Skander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460186/
https://www.ncbi.nlm.nih.gov/pubmed/26054736
http://dx.doi.org/10.1186/s13568-015-0122-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460186/
https://www.ncbi.nlm.nih.gov/pubmed/26054736
http://dx.doi.org/10.1186/s13568-015-0122-7

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