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Fast and reliable production, purification and characterization of heat-stable, bifunctional enzyme chimeras
Degradation of complex plant biomass demands a fine-regulated portfolio of glycoside hydrolases. The LE (LguI/Eco81I)-cloning approach was used to produce two enzyme chimeras CB and BC composed of an endoglucanase Cel5A (C) from the extreme thermophilic bacterium Fervidobacterium gondwanense and an...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Springer Berlin Heidelberg
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460186/ https://www.ncbi.nlm.nih.gov/pubmed/26054736 http://dx.doi.org/10.1186/s13568-015-0122-7 |