Effect of DNA extraction procedure, repeated extraction and ethidium monoazide (EMA)/propidium monoazide (PMA) treatment on overall DNA yield and impact on microbial fingerprints for bacteria, fungi and archaea in a reference soil

Different DNA extraction protocols were evaluated on a reference soil. A wide difference was found in the total extractable DNA as derived from different extraction protocols. Concerning the DNA yield phenol–chloroform–isomyl alcohol extraction resulted in high DNA yield but also in a remarkable co-...

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Detalles Bibliográficos
Autores principales: Wagner, Andreas O., Praeg, Nadine, Reitschuler, Christoph, Illmer, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science B. V 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461152/
https://www.ncbi.nlm.nih.gov/pubmed/26339125
http://dx.doi.org/10.1016/j.apsoil.2015.04.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461152/
https://www.ncbi.nlm.nih.gov/pubmed/26339125
http://dx.doi.org/10.1016/j.apsoil.2015.04.005

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