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Divergence thresholds and divergent biodiversity estimates: can metabarcoding reliably describe zooplankton communities?

DNA metabarcoding is a promising method for describing communities and estimating biodiversity. This approach uses high-throughput sequencing of targeted markers to identify species in a complex sample. By convention, sequences are clustered at a predefined sequence divergence threshold (often 3%) i...

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Detalles Bibliográficos
Autores principales: Brown, Emily A, Chain, Frédéric J J, Crease, Teresa J, MacIsaac, Hugh J, Cristescu, Melania E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461424/
https://www.ncbi.nlm.nih.gov/pubmed/26078859
http://dx.doi.org/10.1002/ece3.1485