Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

Recent use of the CRISPR/Cas9 system has dramatically reduced the time required to produce mutant mice, but the involvement of a time-consuming microinjection step still hampers its application for high-throughput genetic analysis. Here we developed a simple, highly efficient, and large-scale genome...

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Detalles Bibliográficos
Autores principales: Hashimoto, Masakazu, Takemoto, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463957/
https://www.ncbi.nlm.nih.gov/pubmed/26066060
http://dx.doi.org/10.1038/srep11315

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463957/
https://www.ncbi.nlm.nih.gov/pubmed/26066060
http://dx.doi.org/10.1038/srep11315

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