Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

Recent use of the CRISPR/Cas9 system has dramatically reduced the time required to produce mutant mice, but the involvement of a time-consuming microinjection step still hampers its application for high-throughput genetic analysis. Here we developed a simple, highly efficient, and large-scale genome...

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Detalles Bibliográficos
Autores principales: Hashimoto, Masakazu, Takemoto, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463957/
https://www.ncbi.nlm.nih.gov/pubmed/26066060
http://dx.doi.org/10.1038/srep11315
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author Hashimoto, Masakazu
Takemoto, Tatsuya
author_facet Hashimoto, Masakazu
Takemoto, Tatsuya
author_sort Hashimoto, Masakazu
collection PubMed
description Recent use of the CRISPR/Cas9 system has dramatically reduced the time required to produce mutant mice, but the involvement of a time-consuming microinjection step still hampers its application for high-throughput genetic analysis. Here we developed a simple, highly efficient, and large-scale genome editing method, in which the RNAs for the CRISPR/Cas9 system are electroporated into zygotes rather than microinjected. We used this method to perform single-stranded oligodeoxynucleotide (ssODN)-mediated knock-in in mouse embryos. This method facilitates large-scale genetic analysis in the mouse.
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spelling pubmed-44639572015-06-18 Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing Hashimoto, Masakazu Takemoto, Tatsuya Sci Rep Article Recent use of the CRISPR/Cas9 system has dramatically reduced the time required to produce mutant mice, but the involvement of a time-consuming microinjection step still hampers its application for high-throughput genetic analysis. Here we developed a simple, highly efficient, and large-scale genome editing method, in which the RNAs for the CRISPR/Cas9 system are electroporated into zygotes rather than microinjected. We used this method to perform single-stranded oligodeoxynucleotide (ssODN)-mediated knock-in in mouse embryos. This method facilitates large-scale genetic analysis in the mouse. Nature Publishing Group 2015-06-11 /pmc/articles/PMC4463957/ /pubmed/26066060 http://dx.doi.org/10.1038/srep11315 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hashimoto, Masakazu
Takemoto, Tatsuya
Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing
title Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing
title_full Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing
title_fullStr Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing
title_full_unstemmed Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing
title_short Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing
title_sort electroporation enables the efficient mrna delivery into the mouse zygotes and facilitates crispr/cas9-based genome editing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463957/
https://www.ncbi.nlm.nih.gov/pubmed/26066060
http://dx.doi.org/10.1038/srep11315
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AT takemototatsuya electroporationenablestheefficientmrnadeliveryintothemousezygotesandfacilitatescrisprcas9basedgenomeediting

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