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Strategies to improve quantitative assessment of immunohistochemical and immunofluorescent labelling

Binary image thresholding is the most commonly used technique to quantitatively examine changes in immunolabelled material. In this article we demonstrate that if implicit assumptions predicating this technique are not met then the resulting analysis and data interpretation can be incorrect. We then...

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Detalles Bibliográficos
Autores principales: Johnson, Sarah J., Walker, F. Rohan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464180/
https://www.ncbi.nlm.nih.gov/pubmed/26066532
http://dx.doi.org/10.1038/srep10607