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Strategies to improve quantitative assessment of immunohistochemical and immunofluorescent labelling
Binary image thresholding is the most commonly used technique to quantitatively examine changes in immunolabelled material. In this article we demonstrate that if implicit assumptions predicating this technique are not met then the resulting analysis and data interpretation can be incorrect. We then...
Autores principales: | Johnson, Sarah J., Walker, F. Rohan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464180/ https://www.ncbi.nlm.nih.gov/pubmed/26066532 http://dx.doi.org/10.1038/srep10607 |
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