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Vector sequence contamination of the Plasmodium vivax sequence database in PlasmoDB and In silico correction of 26 parasite sequences

We found a 47 aa protein sequence that occurs 17 times in the Plasmodium vivax nucleotide database published on PlasmoDB. Coding sequence analysis showed multiple restriction enzyme sites within the 141 bp nucleotide sequence, and a His6 tag attached to the 3’ end, suggesting cloning vector origins....

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Detalles Bibliográficos
Autores principales: Tao, Zhi-Yong, Sui, Xu, Jun, Cao, Culleton, Richard, Fang, Qiang, Xia, Hui, Gao, Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464627/
https://www.ncbi.nlm.nih.gov/pubmed/26062606
http://dx.doi.org/10.1186/s13071-015-0927-x