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No training required: experimental tests support homology-based DNA assembly as a best practice in synthetic biology

The Registry of Standard Biological Parts imposes sequence constraints to enable DNA assembly using restriction enzymes. Alnahhas et al. (Journal of Biological Engineering 2014, 8:28) recently argued that these constraints should be revised because they impose an unnecessary burden on contributors t...

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Detalles Bibliográficos
Autores principales: Azizi, Afnan, Lam, Wilson, Phenix, Hilary, Tepliakova, Lioudmila, Roney, Ian J, Jedrysiak, Daniel, Power, Alex, Gupta, Vaibhav, Elnour, Nada, Hanzel, Martin, Tzahristos, Alexandra C, Sarwar, Shihab, Kærn, Mads
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464999/
https://www.ncbi.nlm.nih.gov/pubmed/26075023
http://dx.doi.org/10.1186/s13036-015-0006-z