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Restriction-based Multiple-fragment Assembly Strategy to Avoid Random Mutation during Long cDNA Cloning
Long fragment cloning is a challenge for its difficulty in accurate amplifying and tendency to get unwanted mutation. Here we discuss Restriction-based Multiple-fragment Assembly Strategy's advantages and limitations. In this strategy, rather than PCR amplifying the entire coding sequence (CDS)...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466412/ https://www.ncbi.nlm.nih.gov/pubmed/26078793 http://dx.doi.org/10.7150/jca.11779 |