Restriction-based Multiple-fragment Assembly Strategy to Avoid Random Mutation during Long cDNA Cloning

Long fragment cloning is a challenge for its difficulty in accurate amplifying and tendency to get unwanted mutation. Here we discuss Restriction-based Multiple-fragment Assembly Strategy's advantages and limitations. In this strategy, rather than PCR amplifying the entire coding sequence (CDS)...

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Detalles Bibliográficos
Autores principales: Wang, Shang, Chen, Wen, Zhang, Kai, Jiao, Peng, Mo, Lihua, Yang, Xiaoxu, Hu, Xiang, Zhang, Jian, Wei, Chenxi, Xiang, Shuanglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2015
Materias:
Short Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4466412/
https://www.ncbi.nlm.nih.gov/pubmed/26078793
http://dx.doi.org/10.7150/jca.11779

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