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Loss of Foxm1 Results in Reduced Somatotrope Cell Number during Mouse Embryogenesis
FOXM1, a member of the forkhead box transcription factor family, plays a key role in cell cycling progression by regulating the expression of critical G1/S and G2/M phase transition genes. In vivo studies reveal that Foxm1 null mice have a 91% lethality rate at e18.5 due to significant cardiovascula...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468165/ https://www.ncbi.nlm.nih.gov/pubmed/26075743 http://dx.doi.org/10.1371/journal.pone.0128942 |
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author | Calderon, Michael J. Ploegman, Adam G. Bailey, Brock Jung, Deborah O. Navratil, Amy M. Ellsworth, Buffy S. |
author_facet | Calderon, Michael J. Ploegman, Adam G. Bailey, Brock Jung, Deborah O. Navratil, Amy M. Ellsworth, Buffy S. |
author_sort | Calderon, Michael J. |
collection | PubMed |
description | FOXM1, a member of the forkhead box transcription factor family, plays a key role in cell cycling progression by regulating the expression of critical G1/S and G2/M phase transition genes. In vivo studies reveal that Foxm1 null mice have a 91% lethality rate at e18.5 due to significant cardiovascular and hepatic hypoplasia. Thus, FOXM1 has emerged as a key protein regulating mitotic division and cell proliferation necessary for embryogenesis. In the current study, we assess the requirement for Foxm1 in the developing pituitary gland. We find that Foxm1 is expressed in the pituitary at embryonic days 10.5-e18.5 and localizes with markers for active cell proliferation (BrdU). Interestingly, direct analysis of Foxm1 null mice at various embryonic ages, reveals no difference in gross pituitary morphology or cell proliferation. We do observe a downward trend in overall pituitary cell number and a small reduction in pituitary size in e18.5 embryos suggesting there may be subtle changes in pituitary proliferation not detected with our proliferation makers. Consistent with this, Foxm1 null mice have reductions in both the somatotrope and gonadotrope cell populations. |
format | Online Article Text |
id | pubmed-4468165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44681652015-06-25 Loss of Foxm1 Results in Reduced Somatotrope Cell Number during Mouse Embryogenesis Calderon, Michael J. Ploegman, Adam G. Bailey, Brock Jung, Deborah O. Navratil, Amy M. Ellsworth, Buffy S. PLoS One Research Article FOXM1, a member of the forkhead box transcription factor family, plays a key role in cell cycling progression by regulating the expression of critical G1/S and G2/M phase transition genes. In vivo studies reveal that Foxm1 null mice have a 91% lethality rate at e18.5 due to significant cardiovascular and hepatic hypoplasia. Thus, FOXM1 has emerged as a key protein regulating mitotic division and cell proliferation necessary for embryogenesis. In the current study, we assess the requirement for Foxm1 in the developing pituitary gland. We find that Foxm1 is expressed in the pituitary at embryonic days 10.5-e18.5 and localizes with markers for active cell proliferation (BrdU). Interestingly, direct analysis of Foxm1 null mice at various embryonic ages, reveals no difference in gross pituitary morphology or cell proliferation. We do observe a downward trend in overall pituitary cell number and a small reduction in pituitary size in e18.5 embryos suggesting there may be subtle changes in pituitary proliferation not detected with our proliferation makers. Consistent with this, Foxm1 null mice have reductions in both the somatotrope and gonadotrope cell populations. Public Library of Science 2015-06-15 /pmc/articles/PMC4468165/ /pubmed/26075743 http://dx.doi.org/10.1371/journal.pone.0128942 Text en © 2015 Calderon et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Calderon, Michael J. Ploegman, Adam G. Bailey, Brock Jung, Deborah O. Navratil, Amy M. Ellsworth, Buffy S. Loss of Foxm1 Results in Reduced Somatotrope Cell Number during Mouse Embryogenesis |
title | Loss of Foxm1 Results in Reduced Somatotrope Cell Number during Mouse Embryogenesis |
title_full | Loss of Foxm1 Results in Reduced Somatotrope Cell Number during Mouse Embryogenesis |
title_fullStr | Loss of Foxm1 Results in Reduced Somatotrope Cell Number during Mouse Embryogenesis |
title_full_unstemmed | Loss of Foxm1 Results in Reduced Somatotrope Cell Number during Mouse Embryogenesis |
title_short | Loss of Foxm1 Results in Reduced Somatotrope Cell Number during Mouse Embryogenesis |
title_sort | loss of foxm1 results in reduced somatotrope cell number during mouse embryogenesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468165/ https://www.ncbi.nlm.nih.gov/pubmed/26075743 http://dx.doi.org/10.1371/journal.pone.0128942 |
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